What are secondary antibodies?
Secondary antibodies are used to detect primary antibodies.
A primary antibody, generated in one animal species, is detected with a secondary antibody generated in another animal species. Secondary antibodies are usually labeled.
Example of selection:
Primary antibody: Anti-mouse LC3 antibody (generated in rabbits)
Secondary antibody: Anti-rabbit IgG antibody (generated in goats)
Issues to consider when selecting secondary antibodies
Immunized animals
Check the animal species in which the primary antibody to the target molecule is generated.
Label
Select a label suitable for the planned experimental system (fluorescent dye, enzyme, etc.).