Category: Techniques

Simplify your experimental designs and reduce any errors in your data or experiments. When designing your assay, it is crucial to plan and note where you will position your standards, samples, blanks and controls. Typically, most sandwich and competitive ELISA have a seven-point calibration curve. When run in duplicates, that occupies 14 of the 96 […]

What is the significance of the ELISA results for my sample?   Specification Thinks to Remember  Dynamic Range Helps you to see if the kit is able to detect and quantifying the protein amounts you anticipate. Sensitivity This value represents the lowest level of protein that the antibody pair can detect. It should always be less […]

Many scientists prefer to work alone, forming theories, collecting data, and conducting tests. The concept of “networking,” which for many people entails having awkward, superficial discussions with a group of strangers, may be off-putting and unpleasant. Although it may feel awkward and difficult to justify spending time making small chat, it is critical for scientists […]

ELISA are immunoassays widely used in biomarker detection and validation. They have been used in research and clinical settings for more than 40 years, and they allow to quantify, in a simple way, thousands of biomarkers. New ELISAs are being developed every day, either for new biomarkers or new species. They can also be used […]

Introduction KRISHGEN Biosystems is a leading manufacturer of life sciences reagents and kits. Our commitment to marketing products of international standards has accorded it increasing market share worldwide. With man-hours put in for building and understanding in-vitro diagnostics (IVD), KRISHGEN has created a strong knowledge base. KRISHGEN believes that in the knowledge-based society of today, […]

Of all the factors contributing to the performance of a pipette, the most critical are the skill and expertise of the operator. 1. Pre-wet the pipette tip – Aspirate and fully expel an amount of the liquid at least 3 times before aspirating for delivery. Failure to prewet increases evaporation within the tip air space, which […]

Katsumi WAKABAYASHI, Ph. D. Prof. Emer. Gunma University Technical Consultant, Shibayagi Co., Ltd. In usual step for calculation of the assay value of ELISA is to draw a standard curve, absorbance on Y-axis against concentration on X-axis, then to estimate assay value from the absorbance of the sample. EXCEL is really an excellent tool, however, […]