Top 15 Apps for Researchers

We know that academic researchers and PhD scientists are always on the lookout for tools and resources that can help them be more productive and efficient in their work. There now exist a plethora of apps for various tasks available that can help make work easier and more streamlined. Krishgen’s technical team has put together a […]

ELISA Protocol Modifications

The type of antigen immobilization used, as well as the detection method (chemiluminescence, fluorescence, or chemifluorescence) can all be changed in an ELISA protocol. The following section details several ELISA protocol changes and applications. Researchers can extend the variety of detection methods available to them by modifying the ELISA protocol with new technologies. We have […]

GUIDELINES FOR PREPARING ELISA STANDARDS

ELISA standards for accurate sample measurement of the target protein should be carefully prepared to set up a standard curve. A standard curve is usually prepared by assaying a serial dilution of a standard preparation of the target analyte, of which the starting concentration is known When preparing ELISA standards, we’ve included some guidelines to […]

Improving Reproducibility between your ELISA experiments:

Antigen Immobilization: Antibodies binding to certain immobilization antigens can be directly evaluated in immunoassays using bound antigens and appropriate indicators like marked anti-immunoglobulin antibodies. Plastic microtiter plates, glass slides, filter papers, and other similar materials can be used to immobilize antigens.Antigen immobilization varies depending on the type of ELISA assay performed. Direct ELISA – The […]

Immune Complex Optimization in ELISA

The ELISA technique is a powerful and effective immunoassay. The key to an effective ELISA assay, however, is development and optimization. The development of a large immune complex can be difficult, with the success of the test being determined by the failure or incorrect optimization of any component. ELISA signal development can be influenced by […]

Preparation of ELISA Samples

ELISA is an important analytical test in molecular diagnostics, medicine, plant pathology, and basic life science research. ELISA is usually described as the “gold standard” for macromolecule assessment in biological fluids due to its exceptional sensitivity and specificity. The composition, processing, and preservation of the sample being tested are all major elements in enhancing ELISA’s […]

Washing of ELISA Plates

ELISA wash steps are used for a variety of reasons. The washing steps are an important tool to minimize background signal by removing the loose, attached antibody. This helps in improving the signal-to-noise ratio of the analysis. Washing in between steps ensures that only specific (high-affinity) binding events are preserved; result in a signal in […]

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