Simplify your experimental designs and reduce any errors in your data or experiments. When designing your assay, it is crucial to plan and note where you will position your standards, samples, blanks and controls.
Typically, most sandwich and competitive ELISA have a seven-point calibration curve. When run in duplicates, that occupies 14 of the 96 wells available on your plate.
Depending on other parameters you will be running including blanks, controls, and samples, having a clear plate map ensures accuracy and reduces chances of error in your data.
Map out your plate by labelling each well with what you’re adding and the concentration (if known). So, for example, for your standards, you can label the wells as “Std 0”, “Std 20”, “Std 40” etc. Samples can be S1, S2 and so forth.
You can download a printable ELISA plate map here.
