The type of antigen immobilization used, as well as the detection method (chemiluminescence, fluorescence, or chemifluorescence) can all be changed in an ELISA protocol. The following section details several ELISA protocol changes and applications. Researchers can extend the variety of detection methods available to them by modifying the ELISA protocol with new technologies. We have […]
GUIDELINES FOR PREPARING ELISA STANDARDS
ELISA standards for accurate sample measurement of the target protein should be carefully prepared to set up a standard curve. A standard curve is usually prepared by assaying a serial dilution of a standard preparation of the target analyte, of which the starting concentration is known When preparing ELISA standards, we’ve included some guidelines to […]
Improving Reproducibility between your ELISA experiments:
Antigen Immobilization: Antibodies binding to certain immobilization antigens can be directly evaluated in immunoassays using bound antigens and appropriate indicators like marked anti-immunoglobulin antibodies. Plastic microtiter plates, glass slides, filter papers, and other similar materials can be used to immobilize antigens.Antigen immobilization varies depending on the type of ELISA assay performed. Direct ELISA – The […]