Many ELISA kits come with concentrated standards and not ready to use standards. This means we, as user have to reconstitute them to use in the assay. This becomes a critical step as post the running the assay, the interpolation has to be done using the standards. Any shift in the accuracy of the standards […]
Immune Complex Optimization in ELISA
The ELISA technique is a powerful and effective immunoassay. The key to an effective ELISA assay, however, is development and optimization. The development of a large immune complex can be difficult, with the success of the test being determined by the failure or incorrect optimization of any component. ELISA signal development can be influenced by […]
Preparation of ELISA Samples
ELISA is an important analytical test in molecular diagnostics, medicine, plant pathology, and basic life science research. ELISA is usually described as the “gold standard” for macromolecule assessment in biological fluids due to its exceptional sensitivity and specificity. The composition, processing, and preservation of the sample being tested are all major elements in enhancing ELISA’s […]
How To: Get Better Reproducibility In Your Scientific Data
Reproducibility, also known as replicability and or repeatability, is a scientific method in which results obtained by an experiments should be achieved again when it is being performed multiple times under same experimental conditions irrespective of who is running it and where. In research, the ELISA is an invaluable method to detect biological samples and […]