The type of antigen immobilization used, as well as the detection method (chemiluminescence, fluorescence, or chemifluorescence) can all be changed in an ELISA protocol. The following section details several ELISA protocol changes and applications. Researchers can extend the variety of detection methods available to them by modifying the ELISA protocol with new technologies. We have […]
GUIDELINES FOR PREPARING ELISA STANDARDS
ELISA standards for accurate sample measurement of the target protein should be carefully prepared to set up a standard curve. A standard curve is usually prepared by assaying a serial dilution of a standard preparation of the target analyte, of which the starting concentration is known When preparing ELISA standards, we’ve included some guidelines to […]
Improving Reproducibility between your ELISA experiments:
Antigen Immobilization: Antibodies binding to certain immobilization antigens can be directly evaluated in immunoassays using bound antigens and appropriate indicators like marked anti-immunoglobulin antibodies. Plastic microtiter plates, glass slides, filter papers, and other similar materials can be used to immobilize antigens.Antigen immobilization varies depending on the type of ELISA assay performed. Direct ELISA – The […]
How to Perfect your ELISA Standards
Many ELISA kits come with concentrated standards and not ready to use standards. This means we, as user have to reconstitute them to use in the assay. This becomes a critical step as post the running the assay, the interpolation has to be done using the standards. Any shift in the accuracy of the standards […]
Immune Complex Optimization in ELISA
The ELISA technique is a powerful and effective immunoassay. The key to an effective ELISA assay, however, is development and optimization. The development of a large immune complex can be difficult, with the success of the test being determined by the failure or incorrect optimization of any component. ELISA signal development can be influenced by […]
Preparation of ELISA Samples
ELISA is an important analytical test in molecular diagnostics, medicine, plant pathology, and basic life science research. ELISA is usually described as the “gold standard” for macromolecule assessment in biological fluids due to its exceptional sensitivity and specificity. The composition, processing, and preservation of the sample being tested are all major elements in enhancing ELISA’s […]
How To: Get Better Reproducibility In Your Scientific Data
Reproducibility, also known as replicability and or repeatability, is a scientific method in which results obtained by an experiments should be achieved again when it is being performed multiple times under same experimental conditions irrespective of who is running it and where. In research, the ELISA is an invaluable method to detect biological samples and […]
Washing of ELISA Plates
ELISA wash steps are used for a variety of reasons. The washing steps are an important tool to minimize background signal by removing the loose, attached antibody. This helps in improving the signal-to-noise ratio of the analysis. Washing in between steps ensures that only specific (high-affinity) binding events are preserved; result in a signal in […]