What is the significance of the ELISA results for my sample?
| Specification | Thinks to Remember |
| Dynamic Range | Helps you to see if the kit is able to detect and quantifying the protein amounts you anticipate. |
| Sensitivity | This value represents the lowest level of protein that the antibody pair can detect. It should always be less than the standard curve’s lowest point. |
| Precision | This metric indicates how reliable the assay is between runs. A good kit could have a CV of 10%. |
| Standard Curve | Visual evidence that the curve is not low or flat. A poor standard curve indicates that the antibody did not bind correctly or did not capture the protein standard. The trend line’s R2 value should be greater than 0.99. |
| Spike-and-Recovery | Indicates that a target protein can be recovered after being spiked into a matrix. Acceptable values range from 80 to 120 percent. |
| Linearity | Weak linearity of dilution shows that the native sample matrix, sample diluent, and/or standard diluent all have differing effects on protein detection range. |
| Biological Samples | Evidence that the antibody pair recognizes endogenous protein as well as recombinant protein |
| Species Cross-Reactivity | Indicates the amount to which an antibody pair interacts to non-target proteins. This figure should ideally be 5%. |
| International Standard | Validation to an international standard allows for cross-comparison of data sets. This allows you to change from one ELISA supplier to another for the same ELISA kit. |
