ELISA are immunoassays widely used in biomarker detection and validation. They have been used in research and clinical settings for more than 40 years, and they allow to quantify, in a simple way, thousands of biomarkers. New ELISAs are being developed every day, either for new biomarkers or new species. They can also be used […]
ELISA Troubleshooting Guide
Introduction KRISHGEN Biosystems is a leading manufacturer of life sciences reagents and kits. Our commitment to marketing products of international standards has accorded it increasing market share worldwide. With man-hours put in for building and understanding in-vitro diagnostics (IVD), KRISHGEN has created a strong knowledge base. KRISHGEN believes that in the knowledge-based society of today, […]
10 Tips To Improve Your Pipetting Technique
Of all the factors contributing to the performance of a pipette, the most critical are the skill and expertise of the operator. 1. Pre-wet the pipette tip – Aspirate and fully expel an amount of the liquid at least 3 times before aspirating for delivery. Failure to prewet increases evaporation within the tip air space, which […]
How to calculate ELISA assay values by EXCEL
Katsumi WAKABAYASHI, Ph. D. Prof. Emer. Gunma University Technical Consultant, Shibayagi Co., Ltd. In usual step for calculation of the assay value of ELISA is to draw a standard curve, absorbance on Y-axis against concentration on X-axis, then to estimate assay value from the absorbance of the sample. EXCEL is really an excellent tool, however, […]